The Naval Air Warfare Center Training Systems Division (NAWC/TSD), the Air Force Agency for Modeling and Simulation (AFAMS), and the Army Simulation, Training and Instrumentation Command (STRICOM) — the focal point of the nation's simulation and training industry — have their headquarters in the Research Park. Approximately $1.4 billion in federal contracts is awarded each year from the Army and Navy activities in the Research Park.

University organizations, including the Institute for Simulation and Training, are located in the Research Park. For a list of UCF Activities in the Central Florida Research Park, see Central Florida Research Park Tenants.

Other activities include training systems, optics and lasers, medical equipment, behavioral sciences, diagnostic test equipment, cancer research, electric power generation, and wireless communications.